Uji aktivitas antioksidan jus buah mengkudu (Morinda citrifolia) dan perannya sebagai inhibitor advanced glycation end products (AGEs) akibat reaksi glikosilasi
Eko Suhartono, Bambang Setiawan, Edyson,Ramlah
Abstract
Background: Mengkudu is a traditionaI plant with active compound that has antioxidant activity. Diabetes mellitus complication has causative ink with AGEs formation that involves free radical. Objective: To know antioxidant activity of mengkudu and the role to inhibit AGEs formation. Methods: This experiment used pre nd post test control group design. Antioxidant activity was deter-mined by reacting the mengkudu juic with 2,4 dinitrophenylhydrazin (DNPH) and analyzed with spectrophotometer λ = 390 nm. AGEs absort ancy was measured every 48 hours until 20 days with spectrophotometer λ =340 nm. Dicarbonyl level was determined with DNPH method that was developed by Uchida and modified by Sadikin.
Result: Antioxidant activity of 0.25 g/ml mengkudu juice (Morinda citrifolia) was 125.46±27.79%. Beside that, mengkudu juice can inhibit advanced glycation end products (AGEs) formation.
Conclusion: Mengkudu juice can deci case AGEs formation and has antioxidant activity.
sumber : Berkala Ilmu Kedokteran, Vol 37, No 1 (2005)-ojs.lib.unair.ac.id
Eko Suhartono, Bambang Setiawan, Edyson,Ramlah
Abstract
Background: Mengkudu is a traditionaI plant with active compound that has antioxidant activity. Diabetes mellitus complication has causative ink with AGEs formation that involves free radical. Objective: To know antioxidant activity of mengkudu and the role to inhibit AGEs formation. Methods: This experiment used pre nd post test control group design. Antioxidant activity was deter-mined by reacting the mengkudu juic with 2,4 dinitrophenylhydrazin (DNPH) and analyzed with spectrophotometer λ = 390 nm. AGEs absort ancy was measured every 48 hours until 20 days with spectrophotometer λ =340 nm. Dicarbonyl level was determined with DNPH method that was developed by Uchida and modified by Sadikin.
Result: Antioxidant activity of 0.25 g/ml mengkudu juice (Morinda citrifolia) was 125.46±27.79%. Beside that, mengkudu juice can inhibit advanced glycation end products (AGEs) formation.
Conclusion: Mengkudu juice can deci case AGEs formation and has antioxidant activity.
sumber : Berkala Ilmu Kedokteran, Vol 37, No 1 (2005)-ojs.lib.unair.ac.id
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