Uji Aktivitas Antioksidan Noni

Uji aktivitas antioksidan jus buah mengkudu (Morinda citrifolia) dan perannya sebagai inhibitor advanced glycation end products (AGEs) akibat reaksi glikosilasi

Eko Suhartono, Bambang Setiawan, Edyson,Ramlah

Abstract

Background: Mengkudu is a traditionaI plant with active compound that has antioxidant activity. Diabetes mellitus complication has causative ink with AGEs formation that involves free radical. Objective: To know antioxidant activity of mengkudu and the role to inhibit AGEs formation. Methods: This experiment used pre nd post test control group design. Antioxidant activity was deter-mined by reacting the mengkudu juic with 2,4 dinitrophenylhydrazin (DNPH) and analyzed with spectrophotometer λ = 390 nm. AGEs absort ancy was measured every 48 hours until 20 days with spectrophotometer λ =340 nm. Dicarbonyl level was determined with DNPH method that was developed by Uchida and modified by Sadikin.

Result: Antioxidant activity of 0.25 g/ml mengkudu juice (Morinda citrifolia) was 125.46±27.79%. Beside that, mengkudu juice can inhibit advanced glycation end products (AGEs) formation.

Conclusion: Mengkudu juice can deci case AGEs formation and has antioxidant activity.

sumber : Berkala Ilmu Kedokteran, Vol 37, No 1 (2005)-ojs.lib.unair.ac.id